Methylene blue vital staining for Trypanosoma cruzi trypomastigotes and epimastigotes
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منابع مشابه
Methylene blue vital staining for Trypanosoma cruzi trypomastigotes and epimastigotes.
The morphological identification of Trypanosoma cruzi is currently considered to have a high specificity, but its sensitivity, which depends on the volume of the sample examined, is rather low. Trypanosome developmental stages suspended in blood, reduviid feces, and culture media are routinely searched for by means of fresh film examination (about 2 microL). High speed centrifugation of blood s...
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The respiration of T. cruzi epimastigotes was studied using KCN (1 mM) and NaN3 (5 mM) as inhibitors and glucose (33 mM) as substrate. NaN3 inhibits 18% and KCN 58% of the oxygen uptake when T. cruzi cultures enter the stationary phase. The azide-inhibition pattern closely follows endogenous respiration pattern throughout the different growth phases. Titration of the oxygen uptake with cyanide ...
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ژورنال
عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo
سال: 2006
ISSN: 0036-4665
DOI: 10.1590/s0036-46652006000600009